In the present study, the antibacterial activity of a chloroform extract isolated from the fruiting body of medicinal mushroom Ganoderma lucidum (Ling Zhi or Reishi) was investigated. Different concentrations of the crude extract have been used to evaluate its antibacterial activities against Gram-positive bacteria—Bacillus subtilis ATCC 465, Staphylococcus aureus ATCC25923, Enterococcus feacalis ATCC 29737, and Gram-negative bacteria—Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 85327. The results of disk diffusion tests showed that the chloroform extract had growth inhibitory effects on two of the Gram-positive bacteria. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined as well. Both MIC and MBC for Staphylococcus aureus were 8 mg/mL, and for Bacillus subtillis, they were 8 and 16 mg/mL, respectively. To investigate the chloroform extract for its bioactive components, established biochemical methods—the Liebermann-Burchard, Nöller, and Salkowski tests—were used. The results revealed that a variety of lipid derivatives, including sterols and triterpenoid acids, were present in the chloroform extract.
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Keypour et al. (2008) studied this question.