A ferredoxin was isolated from non-photosynthetic tissues of the lower storage root of radish (Raphanus sativus L. var. acantiformis cultivar Miyashige) in a pure form by conventional means. It showed the characteristic features in its absorption spectrum of chloroplast-type ferredoxin. However, amino acid composition and amino (N)- terminal sequence were different from those of radish leaf ferredoxin. Root ferredoxin was able to transfer electrons from dithionite to nitrite reductase [EC 1.7.7.1] isolated from mung bean seedling roots and also to mediate NADP+ photoreduction in spinach broken chloroplasts. It therefore is suggested that a set of distinctive molecular species of ferredoxin is present in non-photosynthetic tissues and functions as a redox mediator in ferredox-independent enzyme systems.
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Wada et al. (1986) studied this question.