Determination of Vitispirane in Wines by Stable Isotope Dilution Assay

The deuterium labeled standard [2H5]-vitispirane was synthesized and utilized in a stable isotope dilution assay for vitispirane in wine. The method is rapid, sensitive, and accurate and for a 5-mL sample of a Cabernet Sauvignon wine, quantitation was reliable down to 500 ng.L−1 with an estimated signal to noise ratio of 3:1. The concentration of vitispirane was measured in 17 red and white commercial wines and was less than the reported threshold. The varietal wines in which vitispirane was detected were Riesling, Chardonnay, Sauvignon blanc, Cabernet Sauvignon, and Pinot noir. The results show that Rieslings have higher concentrations of vitispirane than the other varieties.