Brassinolide (BL),1} the most biologically active and important brassinosteroid (BR), has been assumedto be biosynthesized from castasterone (CS) through a Baeyer-Villiger type of reaction.2)This paper briefly reports evidence for the conversion of CS to BL by using nopaline-type crown gall cells of Catharanthus roseus Don {Vinca rosea L.), which have been known to produce BL and CS.3) C. roseus crown gall cells (V208 strain) were cultured in hormone-free Murashige-Skoog
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Yokota et al. (1990) studied this question.