Wehave already reported the identification of brassinolide (1), castasterone (2), (24S)-24ethylbrassinone (3), and brassinone (4) in the leaves of green tea (Thea sinensis).^Wehavefurther investigated the compoundscontained in the less polar fraction than those of brassinolide and castasterone on normal phase TLC, which showed significant activity in the ricelamina inclination test.In this communication, we describe the structural determination of a newplant growth-promoting brassinosteroid in the fraction by GC-EI-MS.The less polar active fraction obtained previously from tea leaves (65kg) was further purified by reverse phase HPLC (Develosil ODS; 75:25) to yield the two
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Abe et al. (1984) studied this question.