Investigations on Screening of Endophytic Fungal Fractions of Andrographis Paniculata (Burm. F.) Wall Nees. Leaves in Paracetamol and Ethanol Induced Hepatotoxicity

To investigate the hepatoprotective effect of A4EA and A4nB extracts prepared from the endophytic fungi Aspergillus tubingensis strain Cs72 isolated from leaves of Andrographis paniculata on paracetamol and ethanol induced hepatotoxicity. APLF-4 was isolated from Andrographis paniculata leaves by Standard methods to get ethyl acetate A4EA and n butanol A4nB extracts. A4EA and A4nB were screened for hepatoprotective activity against paracetamol and ethanol induced hepatotoxicity at 50 mgkg and 100 mgkg p.o. in rats. Paracetamol and ethanol 50 amp100 mgkg administration significantly elevated the levels of liver marker enzymes as compared to normal control group. A marked decrease in the levels of superoxide dismutase SOD and catalase CAT was also observed. Administration of A4EA and A4nB 50 amp100 mgkg significantly raised the actions of paracetamol and ethanol by decreasing the levels of liver enzymes plt0.001 also increased the concentration of SOD and CAT plt0.001 and decreased lipid peroxidation LPO. APLF-4 was isolated from the leaves of Andrographis paniculata and was identified as Aspergillus tubingensis strain Cs72 by PCR sequential analysis and phylogenetic studies. A4EA and A4nB showed significant hepatoprotective activity in paracetamol and ethanol induced models.