Experimental and Clinical Studies on Unsaturated, Seven-Membered Ring Compounds

The author examined the fungistatic action of unsaturated seven-membered ring compounds i. e. hinokitiol, hinokitiol-natrium, tropolone, 3, 5-dibromotropolone, 3, 5, 7-tribro-motropolone and mercaptotropone on eleven species of cerophilic fungi named by Hans Molish: aspergillus, penicillium etc. The author used the Czapek-Dox medium from which FeSO4 was excluded. He added various quantities of the above mentioned derivative to the medium, in which he culutured the fungi and observed them for two weeks, keeping them in an oven at 25°C.The complete fungistatic concentration of hinokitiol-natrium was 10 γ/cc for penicillium, 70 γ/cc for aspergillus flavus and fusarium, and 20-30 γ/cc (0.107-0.161 millimol) for the other fungi. The fungistatic potency of hinokitiol was at 10-40 γ/cc (0.060-0.240 millimol). It was at 5-40 γ/cc (0.40-0.326 millimol) for tropolone, 5-10 γ/cc (0.036-0.072 millmol) for mercaptotropone. Thus, mercaptotropone was a little stronger than others except on fusarium. 3, 5-dibromotropolone and 3, 5, 7-tribromotropolone had only incomplete fungistatic power at 100 γ/cc (0.357-0.278 millimol). Accordingly, the orders of fungistatic potency of these seven-membered ring compounds on cerophilic fungi are as follow: -1. mercapto-tropone 2. hinokitiol 3. hinckitiol-natrium 4. tropolone. 3, 5-dibromotropolone and 3, 5, 7-tribro motropolone are weaker than the above four compounds.