Key result
NucleoLink surface enables covalent oligonucleotide binding and PCR amplification for ELISA-like amplicon detection.
The NucleoLink surface provides a method for covalent immobilization of primers and subsequent PCR amplification and ELISA-like detection of amplicons.
May support PCR-ELISA nucleic acid detection platforms; leaves open clinical translation pending validation.
The NucleoLink surface is a physically modified, thermostable, optically clear resin. It allows the covalent binding of 5'-phosphorylated oligonucleotides. Target DNA amplification by polymerase chain reaction (PCR) is accomplished by asymmetric amplification on the covalently immobilized primer that develops into immobilized amplicons. A DNA fragment of bovine leukemia virus is used as a model system for the detection of immobilized amplicons by ELISA-like techniques. Covalently bound oligonucleotides are also utilized as capture probe in the hybridization-based signal amplification for detection of an infectious organism.
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Oroskar et al. (1996) studied this question. NucleoLink surface and ELISA-like techniques was evaluated on Detection of immobilized amplicons. The NucleoLink surface allows covalent binding of oligonucleotides and target DNA amplification by PCR for the detection of immobilized amplicons using ELISA-like techniques.
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