An enhanced Glucose Biosensor Modified by Pt/sulfonated-MWCNTs with Layer by Layer Technique

In this work, a glucose biosensor, which was denoted as (GOD/Pt/S-MWCNTs)x/GC biosensor, was constructed successfully by fixing sulfonated MWCNTs immobilized with Pt nanoparticles (Pt/S-MWCNTs) and glucose oxidase (GOD) on the surface of glassy carbon electrode (GCE) with LBL technique. The biosensor constructed by this method was characterized by cyclic voltammogram (CV) and electrochemical impedance spectroscopy (EIS). The detecting performance of the biosensor with different layers was investigated and optimized. The results show that the detection performance of the glucose biosensor reaches the optimum with three layers of GOD/Pt/S-MWCNTs. The detecting performance of the (GOD/Pt/S-MWCNTs)3/GC biosensor to glucose is excellent with the detecting sensitivity of 1.36μA/mM, linear range of 2.5mM and the correlation coefficient of 0.972.