Production of docosahexaenoic acid (DHA; 22:6ω3) from algal oil was achieved using urea complexation technique. The major fatty acids found in algal oil were 22:6, 14:0 and 18:1. As a result of urea complexation, 14:0, 16:0 and 18:1 were eliminated almost completely while DHA was enriched from 47.4 to 97.1% with a process yield of 32.5% of the weight of the original algal oil. The recovery efficiency of DHA was considerably higher and 66.5% of the total DHA present in the original algal oil was found in the DHA concentrate following the urea complexation process.
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Senanayake et al. (2000) studied this question.
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