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January 25, 2021Molecular Medicine ReportsOpen Access

Metabolic remodeling in the right ventricle of rats with severe pulmonary arterial hypertension

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Key result

Severe PAH in rats impairs RV metabolism via decreased TCA cycle and fatty acid oxidation.

  • n=6

Why the study?

Although fatty acid and glucose accumulate in the right ventricle in pulmonary hypertension, the details of metabolic remodeling remain to be elucidated.

Does the Su/Hx model of severe pulmonary arterial hypertension induce metabolic remodeling in the right ventricle of rats?

Population

Male rats

Comparison

SU5416 plus hypoxia followed by normoxia (Su/Hx rats) vs controls

Design

Animal experimental study

Authors

SSSeiichiro SakaoVascular / Pulmonary VascularEKEiryo KawakamiChiba UniversityHSHiroki ShojiTokyo Yamate Medical Center

Discussion

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Overview

Impaired FA oxidation and BCAA dysregulation may reduce TCA activity in severe PH rat RV without glycolysis shift; leaves open therapeutic targets pending human data.

Structured PICO

Does the Su/Hx model of severe pulmonary arterial hypertension induce metabolic remodeling in the right ventricle of rats?

P
Population
6 male Sprague-Dawley rats (3 Su/Hx model, 3 controls) evaluated for metabolic remodeling in the right ventricle.
I
Intervention
SU5416 (20 mg/kg subcutaneous injection) followed by 3 weeks of hypoxia (10% O2) and 5 weeks of normoxia (Su/Hx model)
C
Comparator
Untreated control rats
O
Outcome
Metabolic remodeling in the right ventricle (metabolite levels measured by CE-TOFMS, CE-MS/MS, and LC-TOFMS)surrogate

In a rat model of severe pulmonary arterial hypertension, right ventricular metabolic remodeling is characterized by reduced TCA cycle activity and fatty acid oxidation, alongside dysregulated branched-chain amino acid metabolism, rather than altered glycolysis.

Limitations

  • The total number of rats was extremely small.
  • Lack of mitochondrial staining experiments to confirm differences in mitochondrial form.
  • Analyzed only the right ventricle, not the left ventricle.

Cite This Study

Sakao et al. (2021) studied Severe pulmonary arterial hypertension (n=6). SU5416 followed by hypoxia and normoxia (Su/Hx model) vs. Untreated controls was evaluated on Metabolite concentrations in the right ventricle (TCA cycle intermediates, BCAAs, long-chain acylcarnitines). In a rat model of severe pulmonary arterial hypertension, the right ventricle exhibited a less active TCA cycle, dysregulated branched-chain amino acid metabolism, and decreased fatty acid oxidation.

synapsesocial.com/papers/6aa71ef4b8cfdf2d71d3b795https://doi.org/10.3892/mmr.2021.11866
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