Key result
Radiolabeled 18F-Bodipy-C16 shows high stability and increased brown adipocyte uptake enhanced by beta-adrenergic stimulation.
The newly developed dual-modal fatty acid-based tracer Bodipy-C16 demonstrated high stability and specific uptake in cultured adipocytes, showing potential for future in vivo imaging of brown adipose tissue.
Promising in vitro uptake supports dual-modal BAT tracer development; leaves open in vivo translation and clinical utility.
BACKGROUND: Brown adipose tissue research is in the focus in the field of endocrinology. We designed a dual-modal fluorescent/PET fatty acid based tracer on commercially available Bodipy-C16, which can be synthesized to its corresponding triglyceride and which combines the benefits of fluorescent and PET imaging. METHODS: Bodipy-C16 was coupled to 1,3-diolein resulting in Bodipy-triglyceride. Bodipy-C16 and Bodipy-triglyceride compounds were radiolabeled with 18F using an 18F/19F exchange reaction to yield a dual-modal imaging molecule. Uptake of radiolabeled and non-labeled Bodipy-C16 and Bodipy-triglyceride was analyzed by fluorescence imaging and radioactive uptake in cultured adipocytes derived from human brown adipose tissue and white adipose tissue. RESULTS: Bodipy-C16 and Bodipy-triglyceride were successfully radiolabeled and Bodipy-C16 showed high shelf life and blood plasma stability (99% from 0-4 h). The uptake of Bodipy-C16 increased over time in cultured adipocytes, which was further enhanced after beta-adrenergic stimulation with norepinephrine. The uptake of Bodipy-C16 was inhibited by oleic acid and CD36 inhibitor sulfosuccinimidyl-oleate. The poor solubility of Bodipy-triglyceride did not allow stability or in vitro experiments. CONCLUSION: The new developed dual modal fatty acid based tracers Bodipy-C16 and Bodipy-triglyceride showed promising results to stimulate further in vivo evaluation and will help to understand brown adipose tissues role in whole body energy expenditure.
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Paulus et al. (2017) studied Brown adipose tissue imaging. 18F-Bodipy-C16 and 18F-Bodipy-triglyceride was evaluated on Uptake of radiolabeled and non-labeled Bodipy-C16 in cultured adipocytes. Bodipy-C16 was successfully radiolabeled with 18F, showing high stability and increased uptake over time in cultured brown adipocytes, which was further enhanced by beta-adrenergic stimulation.
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