Summary Human immunoconglutinin was titrated by its reaction with sensitized sheep erythrocytes alexinated with horse complement. IC was shown to be a euglobulin and to be sensitive to the action of 2-mercaptoethanol. On Sephadex G200 chromatography and density gradient ultracentrifugation, its behavior was that of a macroglobulin. IC activity was absorbed by an antiserum to human IgM globulins. In contrast to bovine conglutinin, IC did not require calcium ions for its action, and was not inhibited by N-acetyl-D-glucosamine. Sequential complement fixation at least as far as AgAbC′1423 is required for IC to react with alexinated complexes. Reactivity with autologous complement was demonstrated.
No takes yet. Share an insight, caveat, or question.
Bienenstock et al. (1966) studied this question.