Chemical standardization of kava ( Piper methysticum ) extract using UHPLC-MS/MS and the method of standard addition

Q: What does this research mean for the field?

The method of standard addition effectively corrects for botanical matrix effects, such as ion suppression and signal enhancement, in the quantitative UHPLC-MS/MS analysis of kava extracts. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Ruth N. Muchiri; Natalie R. Thun; Richard B. van Breemen

doi:10.1080/13880209.2026.2727761

September 11, 2026Pharmaceutical BiologyOpen Access

Chemical standardization of kava ( Piper methysticum ) extract using UHPLC-MS/MS and the method of standard addition

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Key Points

To chemically profile and standardize active kavalactones and flavokavains in a botanically authenticated kava (Piper methysticum) extract to support botanical safety research.
Acquired the chemical profile of an authenticated kava extract using ultrahigh-pressure liquid chromatography-high resolution tandem mass spectrometry (UHPLC-HRMS/MS) and identified constituents via reference standards.
Quantified constituents using UHPLC-MS/MS with selected reaction monitoring on a triple quadrupole mass spectrometer, comparing neat-solvent external calibration against the method of standard addition to quantify botanical matrix effects.
Neat-solvent external calibration resulted in 23.2% matrix-induced ion suppression for desmethoxyyangonin.
Signal enhancement was detected for 7,8-dihydrokavain (30.1%) and flavokavain A (43.2%) under external calibration.
The method of standard addition successfully adjusted for electrospray ionization matrix interferences that distort constituent quantification in kava extracts.

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Implication

Laboratory study reveals significant matrix effects during kava extract analysis, highlighting standard addition as essential for accurate dietary supplement standardization.

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Muchiri et al. (2026) studied this question.

synapsesocial.com/papers/6aa7b26d0926e14a848b0cc9 https://doi.org/10.1080/13880209.2026.2727761

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Chemical standardization of kava ( Piper methysticum ) extract using UHPLC-MS/MS and the method of standard addition

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Chemical standardization of kava ( Piper methysticum ) extract using UHPLC-MS/MS and the method of standard addition

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