Biomimetic recombinant humanized type III collagen peptide-RGD loaded alginate hydrogels enhance proliferation of mesenchymal-lineage cells via Integrin α5β1 activation
In vitro study demonstrates that dual-peptide alginate hydrogels promote proliferation in mesenchymal-lineage cells, indicating strong potential for advanced tissue regeneration.
Key Points
To investigate the receptor-level interactions and synergistic biological effects of combining recombinant humanized type-III collagen peptide and RGD within alginate hydrogels on mesenchymal-lineage cells.
Synthesized alginate hydrogels functionalized with RGD (R-HG), recombinant collagen peptide (rCP-HG), or both peptides (CPR-HG).
Evaluated hydrogel swelling, sustained release diffusion, cell proliferation, and morphology via scanning electron microscopy across mesenchymal stem cells (MSCs), human osteoblast-like cells (hFOB), and fibroblasts (FB).
Quantified integrin α5, integrin β1, and collagen-I expression using immunocytochemistry.
Dual-peptide CPR-HG significantly increased cell proliferation compared to single-peptide or unmodified hydrogels, with the most pronounced proliferation observed in hFOB cells.
CPR-HG accelerated cellular integrin α5 and β1 expression across all cell types, exhibiting significantly higher upregulation in MSCs than in hFOB and FB.
Dual-functionalized hydrogels demonstrated sustained peptide diffusion and promoted superior cell adhesion, spreading, and collagen-I expression across all lineages.