Key result
Capillary electrophoresis and MEKC separate doxorubicin derivative FCE 23762 from its diastereomer.
Why the study?
FCE 23762 samples may contain two main impurities, an optical isomer and the degradation product adriamycinone, requiring analytical separation methods.
High performance capillary electrophoresis using two different separation methods provides an efficient and sensitive analysis for the synthetic product of FCE 23762.
Enables sensitive purity analysis of FCE 23762; leaves open clinical translation without validation studies.
High performance capillary electrophoresis was applied to analyse FCE 23762, a new antracycline analogue (doxorubicin derivative) with anti-tumor activity. It has been shown earlier that a FCE 23762 sample may contain two main impurities, one of the optical isomers of FCE 23762 and the most probable degradation product, adriamycinone. The pharmacologically active compound and its diastereomer were resolved in free zone electrophoresis experiments. Adriamycinone, a neutral compound, is baseline separated from the other two (cationic) compounds by micellar electrokinetic chromatography. The use of two different separation methods was advantageous for an efficient and sensitive analysis for the synthetic product of FCE 23762.
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Quaglia et al. (1994) studied this question. High performance capillary electrophoresis was evaluated on Separation of FCE 23762, its optical isomer, and adriamycinone. High performance capillary electrophoresis and micellar electrokinetic chromatography successfully separated the doxorubicin derivative FCE 23762 from its diastereomer and degradation product.
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