Several parameters affecting cold tolerance of winter cereals in artificial freeze tests were examined. Supercooling followed by freezing resulted in death occurring at a higher temperature than when freezing was initiated just below 0 C. The cold tolerance of fully acclimated crowns of winter wheat and a winter rye were reduced an average of 5 C after two thawing and freezing cycles. The duration of freezing in artificial freeze tests has a significant effect on the LD50 of winter cereals. Rapid thawing (2–4 C/min) resulted in death occurring at a higher temperature than slow thawing (0.5–2 C/h).
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Gusta et al. (1977) studied this question.
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