Comparative structural analysis of transcriptionally competent and incompetent rotavirus-antibody complexes
Key result
Fab 2A11/E9 binding alters rotavirus capsid conformation to arrest mRNA synthesis.
Population
Rotavirus particles (four different structural forms, including virus-antibody complexes)
Comparison
Binding of Fab fragments (8H2/G5 and 2A11/E9) vs Native subviral particles and transcriptionally…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May guide rotavirus antiviral design; leaves open translation to human disease.
Structured PICO
P
Population
Rotavirus particles (four different structural forms, including virus-antibody complexes)
I
Intervention
Binding of Fab fragments (8H2/G5 and 2A11/E9)
C
Comparator
Native subviral particles and transcriptionally incompetent mature particles
O
Outcome
Conformation of the capsid and efficiency of mRNA production/transcriptionsurrogate
Continuous translocation of nascent mRNA through the rotavirus capsid is critical for efficient transcript elongation, and blockage causes premature termination.
Cite This Study
Lawton et al. (1999) studied Rotavirus infection (basic science). Fab (8H2/G5) and Fab (2A11/E9) binding vs. Native subviral particle was evaluated on mRNA production and transcript elongation. Binding of Fab 2A11/E9 introduces conformational changes in the rotavirus capsid that arrest mRNA synthesis, indicating continuous translocation of nascent mRNA is critical for transcript elongation.