Key result
CD46 SCR IV exchange reduces measles virus binding and abolishes receptor down-regulation while supporting entry.
Population
Transfected CHO cell lines stably expressing chimeric proteins (x3DAF and x4DAF) or wild-type CD46
Comparison
Expression of chimeric proteins x3DAF and x4DAF vs Wild-type CD46 (I-II-III-IV)
Design
Preclinical
Authors
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CD46 SCR IV modulates measles virus binding in cell models without blocking entry; hypothesis-generating for receptor mapping and requires in vivo confirmation.
CD46 SCR IV is required for optimal measles virus binding and receptor down-regulation, but not strictly required for virus entry.
Christiansen et al. (2000) studied Measles virus infection (in vitro). Chimeric proteins x3DAF and x4DAF (exchange of CD46 SCR III and IV) vs. Wild-type CD46 was evaluated on Measles virus binding, infection, fusion, and receptor down-regulation. Exchange of CD46 SCR IV (x4DAF) significantly decreased measles virus binding and abolished receptor down-regulation, although the cells still supported virus entry.
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