Programmed cell death-related ABI1 is a critical mediator of abdominal aortic aneurysm

Figures

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  Fig. 1. Correlation between CD score and 12 kinds of PCD scores in GSE98278

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  Fig. 2. WGCNA for CD score-related gene module in GSE98278. A Relationship between scale-free topology model fit, mean connectivity, and soft threshold. B Waterfall plot shows the clustering results of WGCNA-derived module genes. C Correlation between CD score and gene modules. D Correlation between gene significance and module membership in the turquoise module

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  Fig. 3. Enriched GO–BP, GO–CC, GO–MF, and KEGG pathways based on turquoise module genes in GSE98278

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  Fig. 4. Identification of AAA-related feature genes. A Volcano plot shows the DEGs between AAA and normal samples in GSE47472. B Venn plot shows the intersected genes between DEGs and turquoise module genes. C Vlnplot shows the expression patterns of 12 intersected genes in AAA and normal samples in GSE47472

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  Fig. 5. Immune features of ABI1. A LASSO regression analysis on 12 intersected genes. B Coefficients of 12 intersected genes in the LASSO model. C Heatmap shows the correlation between ABI1 and immune cells. D Enriched GSEA pathways related to ABI1

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  Fig. 6. In vitro validation on ABI1. A Western blot assay shows the protein expression of Vimentin in four groups. B Western blot assay shows the protein expression of BAX in four groups. C Western blot assay shows the protein expression of BCL-2 in four groups. D Western blot assay shows the protein expression of Caspase3 in four groups. E Western blot assay shows the protein expression of PARP1 in four groups. F Western blot assay shows the protein expression of MMP2 in four groups. G Apoptosis assay shows the cell apoptosis rates in four groups. H Statistical analysis of apoptosis assay. I Transwell assay shows the migrated cells in four groups. J Transwell assay shows the migrated cells in four groups

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  Fig. 7. Correlation between ABI1 and 12 kinds of PCD scores in GSE98278