Key result
Bovine calf serum shows no difference versus fetal bovine serum for aortic valve cell activity.
Why the study?
The efficacy of less-costly alternative sera compared to fetal bovine serum for culturing porcine aortic valve interstitial cells has not been documented.
Does bovine calf serum, bovine growth serum, or newborn calf serum maintain cellular activity comparable to fetal bovine serum in cultured porcine aortic valve interstitial cells?
Does bovine calf serum, bovine growth serum, or newborn calf serum maintain cellular activity comparable to fetal bovine serum in cultured porcine aortic valve interstitial cells?
Bovine calf serum is a suitable, less-costly alternative to fetal bovine serum for the 2-D and 3-D culture of porcine aortic valve interstitial cells for tissue engineering.
May favor fetal/newborn calf serum over bovine growth serum for engineered tissue quality; leaves open cost-effective serum substitution in primary cell bioprocesses.
Unlike established cell lines used in the biotechnology industry, primary cells used in tissue engineering require culture media to be supplemented with serum. The most common serum is fetal bovine serum (FBS); however, FBS is expensive, negatively affecting process economics. Less-costly alternative sera are commercially available, but their efficacy has not been documented. Therefore, bovine calf serum (BCS), bovine growth serum (BGS), and newborn calf serum (NCS) were compared with FBS. Porcine aortic valve interstitial cells (VICs) were cultured as 2-dimensional (2-D) monolayers or as 3-dimensional (3-D) collagen gels using medium supplemented with 10% FBS, BGS, BCS, or NCS. No significant difference was seen in cellular activity between VICs cultured in BCS and those cultured in FBS in 2-D cultures, whereas cells cultured in BGS and NCS had significantly lower specific growth rates coupled with markedly higher metabolic activity than cells cultured in FBS. No statistically significant differences were seen in cellular activity between any of the sera when cells were cultured in 3-D constructs. In conclusion, BCS is a suitable alternative to FBS for the 2-D and 3-D culture of VICs, which may be used to develop a tissue-engineered valve.
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Bond et al. (2007) studied this question. Bovine calf serum (BCS), bovine growth serum (BGS), and newborn calf serum (NCS) vs. Fetal bovine serum (FBS) was evaluated on Cellular activity (specific growth rates and metabolic activity). Bovine calf serum (BCS) showed no significant difference in cellular activity compared to fetal bovine serum (FBS) in 2-D and 3-D cultures of porcine aortic valve interstitial cells.
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