Key result
New HPLC-RIA method successfully measures plasma angiotensins I, II, and III, detecting expected changes with captopril.
Why the study?
A precise and sensitive method to measure endogenous angiotensins I, II, and III in human plasma was needed to accurately assess their plasma levels.
The described HPLC and RIA method provides a sensitive and reproducible way to precisely measure plasma levels of endogenous angiotensins I, II, and III.
May enable precise angiotensin profiling in research; leaves open validation and clinical utility in larger studies.
We measured endogenous angiotensins (ANGs) I, II&III using a system of extraction by Sep-Pak column followed by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). An excellent separation of ANGs was obtained by HPLC. The recovery of ANGs I, II&III was 80-84%, when these authentic peptides were added to 6 ml of plasma. The coefficient of variation of the ANGs was 0.04-0.09 for intra-assay and 0.08-0.13 for inter-assay, thereby indicating a good reproducibility. Plasma ANGs I, II&III measured by this method in 5 normal volunteers were 51,4.5 and 1.2 pg/ml. In the presence of captopril, ANGs II&III decreased by 84% and 77%, respectively, while ANG I increased 5.1 times. This method is therefore useful to assess the precise levels of plasma ANGs.
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Kawamura et al. (1987) studied Normal volunteers (n=5). Captopril was evaluated on Plasma levels of ANGs I, II, and III. A new HPLC-RIA method successfully measured plasma angiotensins I, II, and III in 5 normal volunteers, demonstrating expected changes in the presence of captopril.
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