A polishable amperometric biosensor for bilirubin

An amperometric enzyme electrode for bilirubin based on the incorporation of bilirubin oxidase and horseradish peroxidase within a graphite‐epoxy matrix is reported. The resulting bienzyme electrodes are renewable (by polishing) and offer low‐potential detection of bilirubin. The limit of detection is 4 × 10−6 M and the response is linear up to 1 × 10−4 M. The influence of various experimental variables is described. The surface‐to‐surface reproducibility is 14% (in terms of relative standard deviation). The fabrication scheme is simple and the response times are short. The prospects of using amperometric bilirubin electrodes for diagnostic work are discussed.