What question did this study set out to answer?

Determine whether human platelets labeled at two discrete densities of biotin maintain normal in vitro function and can be simultaneously tracked in vivo in an animal circulation model.

February 13, 2021Transfusion

Human platelets labeled at two discrete biotin densities are functional in vitro and are detected in vivo in the murine circulation: A promising approach to monitor platelet survival in vivo in clinical research

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Authors

CRCatherine RavanatÉtablissement Français du Sang APAnaïs PongérardInserm MFMonique FreundMayo Clinic in Arizona

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Implication

Preclinical study demonstrates successful tracking of dual-density biotinylated human platelets in mice, highlighting a non-radioactive alternative for clinical platelet survival research.

Key Points

Determine whether human platelets labeled at two discrete densities of biotin maintain normal in vitro function and can be simultaneously tracked in vivo in an animal circulation model.
Manufactured injectable-grade human platelets under sterile conditions using clinical-grade buffers and GMP-grade Sulfo-NHS-Biotin at 1.2 μg/mL and 10 μg/mL.
Evaluated platelet morphology, activation markers, apoptotic signals, aggregation, secretion, and surface glycoprotein expression in vitro.
Injected labeled platelets into severe immunodeficient mice and analyzed survival kinetics and population distinction using ex vivo flow cytometry.
Human platelets labeled with 1.2 or 10 μg/mL Sulfo-NHS-Biotin preserved normal ultrastructure, surface glycoproteins, and activation/secretion capacity without premature apoptosis.
Flow cytometry successfully distinguished both biotinylated platelet cohorts from each other and from unlabeled recipient mouse platelets.
Circulating survival kinetics of the biotin-labeled human platelets in mice matched the survival observed for unlabeled human platelets.

Cite This Study

Ravanat et al. (2021) studied this question.

synapsesocial.com/papers/6aa88722b4a7d5c7d84d0752 https://doi.org/10.1111/trf.16312

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February 13, 2021Transfusion

Human platelets labeled at two discrete biotin densities are functional in vitro and are detected in vivo in the murine circulation: A promising approach to monitor platelet survival in vivo in clinical research

View Full Paper

Ask AI

Bookmark

Authors

CRCatherine RavanatÉtablissement Français du Sang APAnaïs PongérardInserm MFMonique FreundMayo Clinic in Arizona

Discussion

Member takes

Implication

Preclinical study demonstrates successful tracking of dual-density biotinylated human platelets in mice, highlighting a non-radioactive alternative for clinical platelet survival research.

Key Points

Determine whether human platelets labeled at two discrete densities of biotin maintain normal in vitro function and can be simultaneously tracked in vivo in an animal circulation model.
Manufactured injectable-grade human platelets under sterile conditions using clinical-grade buffers and GMP-grade Sulfo-NHS-Biotin at 1.2 μg/mL and 10 μg/mL.
Evaluated platelet morphology, activation markers, apoptotic signals, aggregation, secretion, and surface glycoprotein expression in vitro.
Injected labeled platelets into severe immunodeficient mice and analyzed survival kinetics and population distinction using ex vivo flow cytometry.
Human platelets labeled with 1.2 or 10 μg/mL Sulfo-NHS-Biotin preserved normal ultrastructure, surface glycoproteins, and activation/secretion capacity without premature apoptosis.
Flow cytometry successfully distinguished both biotinylated platelet cohorts from each other and from unlabeled recipient mouse platelets.
Circulating survival kinetics of the biotin-labeled human platelets in mice matched the survival observed for unlabeled human platelets.

Cite This Study

Ravanat et al. (2021) studied this question.

synapsesocial.com/papers/6aa88722b4a7d5c7d84d0752 https://doi.org/10.1111/trf.16312

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