Effect of ortho‐dihydroxyphenols on growth and protein pattern of callus cultures from Prunus avium

Excised shoot discs from Prunus avium L. were cultivated on a modified Murashige and Skoog medium for 4 weeks under long day conditions. The basal medium was supplemented with IAA and benzylaminopurine. Further addition of 5.7 × 10−5 resp, 11.4 × 10−5M catechin and 4.25 × 10−5 resp. 8.5 × 10−5M chlorogenic acid resulted in a highly significant promotion of callus proliferation. Proteins and enzymes were separated by isoelectric focusing. The supply of both polyphenols resulted in a higher number and intensity of protein bands including esterases as compared to controls.