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Three previously known ingenol esters, milliamines A-C and four new ingenol esters, milliamines D-G, all containing anthraniloyl tripeptides in various positions of ingenol, were isolated from roots and stems of Euphorbia milii , using a combination of counter-current distribution, preparative TLC and preparative HPLC. All the esters were fully characterised and showed irritant activity on the mouse ear. Neither milliamine A nor milliamine C (the most irritant ester) was a tumour promoter on mouse back skin. Key Word Index Euphorbia milii - Euphorbiaceae - Ingenol Esters - Irritation 1 For part V of the series, see loc. cit. [1].
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Marston et al. (1983) studied this question.
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