N-Bromosuccinimide cleavage reaction on cerexin A liberated allo-isoleucine. Treatment with conc. hydrochloric acid cleaved the antibiotic into two peptide fragments selectively at gamma-hydroxylysine residue. Deacylation with an enzyme preparation from Pseudomonas sp. afforded deacyl cerexin A. The amino acid sequences of these peptide fragments were examined by Edman degradation. From all the results, the entire amino acid sequence of cerexin A was deduced.
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SHOJI et al. (1975) studied this question.