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Rat liver mitochondrial lipase has been purified 77-fold.The procedure involves solubilizing the enzyme by homogenizing mitochondria in Triton X-100 and sonication.Purification is achieved by gel filtration on Sephadex G-50 and G-ZOO.This enzyme has a molecular weight greater than 800,000.Hydrolysis of various triglycerides by this lipase increases as the chain length of the esterified fatty acids decreases.Hydrolysis of unsaturated glycerides is in the order monoglyceride > diglyceride > triglyceride.Hydrolysis of palmitic acid glycerides is in the order monopalmitin > tripahnitin > dipalmitin.With respect to the unsaturation of the esteritied fatty acids of glycerides the hydrolysis increases as the degree of unsaturation increases.The pH optimum for this lipase is between 7.5 and 8.0.Triton X-100, calcium chloride, sodium fluoride, diisopropyl fluorophosphate, and ammonium chloride inhibit the mitochondrial lipase.The characteristics of the mitochondrial lipase are compared to those of lipases from other sources.
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Claycomb et al. (1971) studied this question.
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