Substitution of egg yolk for the mother hen's serum does away with the cumbersome procedure of bleeding the birds for antibody tests. The level of RSVA in yolk reflects the serum antibody level but is usually somewhat lower. Furthermore, substitution of egg yolk for the mother hen's serum in an indirect immunofluorescence procedure provides a simple, rapid, reliable and relatively inexpensive testing system. Flocks of birds can be easily examined and in most cases the test results are available the same day.
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Aulisio et al. (1967) studied this question.
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