Detection of Matrix Metalloproteinase 2 by a Polypeptide Cleavable Split-Type Sensor Based on Atomically Precise Bimetallic Nanocluster Au3Ag5(MSA)3

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In this study, a split-type electrochemiluminescence (ECL) sensor based on polypeptide cleavage was proposed for the sensitive detection of matrix metalloproteinase 2 (MMP 2). The bimetallic nanoclusters were protected by mercaptosuccinic acid (MSA), named Au 3 Ag 5 (MSA) 3, serving as a signal probe. Owing to the synergistic effect of the bimetal composition, the clusters exhibited enhanced ECL properties. Polypeptide chains (PLGVR) that can be specifically cleaved by MMP 2 were selected from the MMP 2 domain. Additionally, the antifouling segment (PPEKEK) and the binding segment (CCC) were incorporated into the polypeptide chain design. In the presence of MMP 2, a specific cleavage site on the polypeptide was targeted, resulting in the detachment of Au 3 Ag 5 (MSA) 3 and subsequent generation of an ECL signal. Compared to conventional detection methods, this approach exhibited enhanced sensitivity and improved stability. This method provides a valuable reference for detecting members of the matrix metalloproteinase family and holds promising prospects for future development.