Characterization of Hemoglobin Immobilized in MgAl-Layered Double Hydroxides by the Coprecipitation Method

Hemoglobin was immobilized in Mg(2)Al-Layered Double Hydroxides (LDH) by coprecipation method at pH 9.0. Interactions between Hb and LDH particles were investigated by X-ray diffraction patterns, FTIR, UV-vis, circular dichroism, and fluorescence spectroscopies. Morphology and porosity of Mg(2)Al-Hb(cop) biohybrid are analyzed from SEM and TEM images and permeability measurement. The direct electron transfer of immobilized Hb was studied by cyclic voltammetry, and the electrocatalytic activity was evaluated at glassy carbon modified with this Mg(2)Al-Hb(cop) biohybrid. Even though the percentage of electroactive Hb was less than 2%, this bioelectrode showed a low detection limit (1.5 x 10(-8) M) and a very high sensitivity (37 A/M cm(2)) for the amperometric detection of H(2)O(2).