Curcumin Confer Radiosensitizing Effect in Breast Cancer Cell Lines via Growth and Motility Inhibition

Background and Objective: Breast cancer is a major cause of morbidity and mortality in economically developing countries such as India.Evidential research is explained about the anticancer properties.The present study investigated curcumin along with the γ-irradiation effect on two different breast cancer cell lines.Materials and Methods: MDA-MB-231 and MCF-7 cell was irradiated with gamma rays prior to the curcumin treatment.A 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, clonogenic assays and wound healing assays were performed.The results were analyzed with a one-way analysis of variance (ANOVA) with the Bonferroni multiple comparison post-test, using In Stat software version 3.00.Results: The results of the MTT assay showed a statistical significance with p>0.001, similarly, clonogenic assay revealed that the curcumin reduced the proliferation rate of cells.Correspondingly, wound healing assay resulted in control of the cell-cell interaction and cell migration at two different time points.Conclusion: In conclusion, curcumin along with a lower dose (8 Gy) of radiation acted as the best combinatorial drug treatment for breast cancer cell lines.Our findings put up a rationale for further clinical/preclinical analysis of combination treatments with a lower dose and fewer side effects in breast cancer.