An Analysis of the Serum Metabolomic Profile for the Radiomitigative Effect of the Thrombopoietin Receptor Agonist Romiplostim in Lethally Whole-Body-Irradiated Mice

Figures

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  Figure 1. Effect of TBI on the metabolic profile in serum. Boxplots of metabolite were presented. The black dots represent the normalized value of metabolite. The notch indicates 95% confidence interval around the median. Asp—aspartate; Trp—tryptophan.

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  Figure 2. Feature of metabolite in the serum from TBI mice. (A) Variable importance in projection (VIP) scores of the partial-least-squares discriminant analysis (PLS-DA) in TBI and control mice. The red and blue dots indicate the corresponding metabolite level was increased and decreased by TBI, respectively. (B) A pathway analysis combining pathway enrichment and pathway topology analyses of metabolites whose levels were significantly changed by TBI. The x-axis marks the pathway impact, and the y-axis represents the pathway enrichment. Each node marks a pathway, with larger sizes and darker colors representing higher pathway impact values and greater pathway enrichment. Asp—aspartate; Trp—tryptophan.

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  Figure 3. PLS-DA plots of the serum metabolome. PLS-DA plot shows separation for study groups control, TBI and TBI + RP based on metabolic profiles.

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  Figure 4. Radiomitigative effects of RP on the disruption of metabolome profiling and pathway by TBI. (A) An IPA analysis of the top canonical pathways in TBI and TBI + RP is represented. Colors indicate higher or lower Z scores. (B) Raindrop illustration of metabolite patterns showed fold changes between TBI and control or TBI + RP and TBI. The scale bar represents the normalized intensity of metabolites, where blue indicates a decrease/low and red indicates an increase/high. The size of the drop represents the −log10(p-value); Asp—Aspartate. (C) Boxplots of metabolite were presented. The black dots represent the normalized value of metabolite. The notch indicates 95% confidence interval around the median.