An analytical method was based on the aldehyde‐bisulfite (separated by HPLC) reaction with o ‐phthalaldehyde in the presence of ammonium acetate, producing a fluorescent compound. Detection limits of alkanalbisulfite (from formaldehyde‐ to octylaldehyde‐bisulfite) and 2‐alkenalbisulfite (from trans ‐2‐hexenal‐ to trans ‐2‐nonenal‐bisulfite) ranged from 5 nM to 25 nM. The method was less sensitive for free sulfite (detection limit 100 μM) than for aldehyde‐bisulfite. Increasing acetaldehyde‐bisulfite with addition of 25 mM acetaldehyde to a beer sample showed free sulfite in the beer. Free sulfite and acetaldehyde‐bisulfite were detected in commercial beers but other aldehyde‐bisulfites were not.
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Kaneda et al. (1996) studied this question.
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