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Summary. A modification of Bergersen's synthetic medium gave improved growth of Rhizobium. When potassium was included in the medium the biotin concentration could be decreased from the recommended 150–250 to 0·5 μg/l. Growth of 15 strains from 6 Rhizobium spp. was compared in the original and modified media and the responses of those strains to biotin, thiamine and calcium pantothenate noted.
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Marie T. Sherwood (1970) studied this question.
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