Genomic analysis reveals horizontal gene transfer of GH32 enzymes in planctomycetes, highlighting diverse mechanisms of bacterial carbohydrate utilization.
Key Points
Investigate the genetic diversity, domain architecture, and evolutionary trajectory of potential β-fructosidases across the bacterial phylum Planctomycetes.
Analyzed genomic sequences from planctomycetes to profile glycoside hydrolase genes linked to fructan degradation.
Conducted phylogenetic evaluations and protein domain architecture analyses focusing on glycoside hydrolase family GH32 and carbohydrate-binding module family CBM38.
Identified subfamily 32b of the GH32 glycoside hydrolase family as the key enzymatic driver of fructan utilization in planctomycetes.
Demonstrated multiple lateral gene transfer events of β-fructosidase genes within Planctomycetes and across diverse bacterial phyla.
Uncovered structural variations in planctomycetal β-fructosidases, specifically the selective presence or absence of the CBM38 substrate-binding domain.