What does this research mean for the field?

Combining fluoride with plant polyphenols significantly modifies the proteome of the dentine pellicle by lowering the overall abundance of proteins and proteases, which explains their protective mechanism against dentine demineralization. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To characterize the proteomic profile of in vitro human dentine pellicles before and after modification with plant polyphenols and fluoride formulations.

September 15, 2026Clinical Oral InvestigationsOpen Access

Proteomic profile of the dentine pellicle modified with plant polyphenols and fluoride

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Authors

TCThiago Saads Carvalho TBTommy Baumann MBMarília Afonso Rabelo Buzalaf

Discussion

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Overview

In vitro study reveals that combining plant polyphenols with fluoride alters the dentine pellicle proteome and reduces protease abundance, suggesting a mechanism against dentine demineralization.

Key Points

To characterize the proteomic profile of in vitro human dentine pellicles before and after modification with plant polyphenols and fluoride formulations.
Assigned 135 human dentine specimens with saliva-formed pellicles to one of nine modification solutions: deionized water, NaF, SnCl2/NaF/AmF, or blueberry, green tea, or grape seed extracts (2 mg/ml) with or without 500 ppm fluoride.
Harvested acquired pellicles using sodium dodecyl sulphate following a second human saliva incubation period.
Identified and quantified pellicle proteins across all groups using liquid chromatography-tandem mass spectrometry after tryptic digestion.
Identified a total of 382 proteins across all experimental groups.
Observed 12 and 14 exclusive proteins following treatment with NaF and SnCl2/NaF/AmF, respectively, compared to 4 to 6 exclusive proteins for polyphenol extracts alone.
Demonstrated that combining fluoride with green tea or grape seed extracts resulted in the fewest exclusive proteins and notably lowered the abundance of proteases.

Cite This Study

Carvalho et al. (2026) studied this question.

synapsesocial.com/papers/6aa913609013453be30a12db https://doi.org/10.1007/s00784-026-07097-y

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September 15, 2026Clinical Oral InvestigationsOpen Access

Proteomic profile of the dentine pellicle modified with plant polyphenols and fluoride

View Full Paper

Ask AI

Bookmark

Authors

TCThiago Saads Carvalho TBTommy Baumann MBMarília Afonso Rabelo Buzalaf

Discussion

Member takes

Overview

In vitro study reveals that combining plant polyphenols with fluoride alters the dentine pellicle proteome and reduces protease abundance, suggesting a mechanism against dentine demineralization.

Key Points

To characterize the proteomic profile of in vitro human dentine pellicles before and after modification with plant polyphenols and fluoride formulations.
Assigned 135 human dentine specimens with saliva-formed pellicles to one of nine modification solutions: deionized water, NaF, SnCl2/NaF/AmF, or blueberry, green tea, or grape seed extracts (2 mg/ml) with or without 500 ppm fluoride.
Harvested acquired pellicles using sodium dodecyl sulphate following a second human saliva incubation period.
Identified and quantified pellicle proteins across all groups using liquid chromatography-tandem mass spectrometry after tryptic digestion.
Identified a total of 382 proteins across all experimental groups.
Observed 12 and 14 exclusive proteins following treatment with NaF and SnCl2/NaF/AmF, respectively, compared to 4 to 6 exclusive proteins for polyphenol extracts alone.
Demonstrated that combining fluoride with green tea or grape seed extracts resulted in the fewest exclusive proteins and notably lowered the abundance of proteases.

Cite This Study

Carvalho et al. (2026) studied this question.

synapsesocial.com/papers/6aa913609013453be30a12db https://doi.org/10.1007/s00784-026-07097-y

View Full Paper

Ask AI

Bookmark