Distinct Response of Bacterial and Fungal Communities to Nitrogen Decrement and Green Manure Incorporation Under Slow-Release Nitrogen Fertilizer Application
Field experiment demonstrates that green manure mitigates growth loss and suppresses pathogens under reduced nitrogen in paddy rice, highlighting a sustainable soil management strategy.
Key Points
To evaluate how reduced slow-release nitrogen application alone and paired with green manure influences rice growth, rhizosphere properties, and soil microbial communities.
Conducted a field experiment in paddy rice under slow-release nitrogen fertilizer management.
Evaluated three treatments: normal nitrogen rate (RN), 20% nitrogen reduction (RND), and 20% nitrogen reduction combined with green manure incorporation (RNDG).
Measured rice growth parameters, rhizosphere soil nutrients, and deterministic versus stochastic community assembly processes for bacteria and fungi.
Nitrogen reduction (RND) negatively affected rice growth, but combining reduction with green manure (RNDG) mitigated growth loss while decreasing plant pathogenic fungi.
Fungal communities displayed greater sensitivity to treatments than bacterial communities, with deterministic assembly processes strengthening for bacteria under RND and RNDG but decreasing for fungi under RND.
Bacterial community variation correlated primarily with soil ammonium and nitrate levels, whereas fungal communities were mainly linked to available soil and plant phosphorus.