The essential oil obtained from the aerial parts of Ajuga chamaecistus Ging. subsp. tomentella Rech. f..was analyzed by using GC and GC-MS. Forty four compounds representing 95.13 % of the oil were identified. The main ones were thymol (34.45 %), exo-fenchol (15.58 %), β-pinene (8.26 %), 1-octen-3-ol (5.92 %), α-terpineol (3.88 %), 2-hexanol (3.85 %), α-thujene (2.66 %), and α-pinene (2.54 %). The oil was rich in oxygenated monoterpenes. Antibacterial activity of the essential oil was determined using disc diffusion method against both Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria. The experiments showed that the pure essential oils had no antibacterial activity against Gram-positive and Gram-negative bacteria at 25 μl/disc.
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