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July 1, 1987Applied and Environmental MicrobiologyOpen Access

Identification of Lotus Rhizobia by Direct DNA Hybridization of Crushed Root Nodules

James E Cooper Anthony J. Bjourson John F. Thompson

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Abstract

Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did not occur. Results were comparable to those from colony hybridization.

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Cooper et al. (1987) studied this question.

synapsesocial.com/papers/6aa925056dc8754dbe62e0cd https://doi.org/10.1128/aem.53.7.1705-1707.1987

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