What question did this study set out to answer?

To determine the biological function of the novel mammalian mitochondrial protein 9030617O03Rik and examine its role in cardiac amino acid metabolism.

March 7, 2017Scientific ReportsOpen Access

D-Glutamate is metabolized in the heart mitochondria

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Authors

MAMakoto AriyoshiKyoto Prefectural University of Medicine MKMasumi KataneKitasato Institute Hospital KHKenji HamaseKyushu University

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Implication

Laboratory study reveals novel D-glutamate cyclase activity in mouse heart mitochondria, indicating an active pathway for D-glutamate metabolism linked to cardiac health.

Key Points

To determine the biological function of the novel mammalian mitochondrial protein 9030617O03Rik and examine its role in cardiac amino acid metabolism.
Evaluated 9030617O03Rik expression in heart failure models and conducted genomic homology analyses against bacterial enzymes.
Profiled free amino acid concentrations in cardiac tissue using 9030617O03Rik-deficient mice.
Performed molecular cloning and biochemical assays to characterize enzymatic activity and substrate conversion.
9030617O03Rik expression showed marked reduction under heart failure conditions.
Heart tissues of 9030617O03Rik-deficient mice exhibited significant accumulation of D-glutamate.
Enzyme assays identified 9030617O03Rik as a D-glutamate cyclase that metabolizes D-glutamate into 5-oxo-D-proline in mammalian mitochondria.

Cite This Study

Ariyoshi et al. (2017) studied this question.

synapsesocial.com/papers/6aa929c4bd00adaa8902c37b https://doi.org/10.1038/srep43911

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Implication

Laboratory study reveals novel D-glutamate cyclase activity in mouse heart mitochondria, indicating an active pathway for D-glutamate metabolism linked to cardiac health.

D-Glutamate is metabolized in the heart mitochondria

Discussion

Member takes

Implication

Key Points

Cite This Study

D-Glutamate is metabolized in the heart mitochondria

Discussion

Member takes

Implication

Key Points

Cite This Study

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