Key result
ATeam technology detects viral cytopathic effects via ATP changes as early as 3 hours post-infection.
Why the study?
ATP is involved in cellular processes including the viral life cycle and can indicate early cytopathic effects, prompting evaluation of an ATP probe to detect these effects.
p-value: p=<0.01
ATeam technology enables the detection of early signs of viral infection by measuring ATP-based cytopathic effects as early as 3 hours post-infection, preceding morphological changes.
May accelerate in vitro viral assays; leaves open clinical diagnostic validation.
Adenosine 5'-triphosphate (ATP), the major energy currency of the cell, is involved in many cellular processes, including the viral life cycle, and can be used as an indicator of early signs of cytopathic effect (CPE). In this study, we demonstrated that CPE can be analyzed using an FRET-based ATP probe named ATP indicator based on Epsilon subunit for Analytical Measurements (ATeam). The results revealed that as early as 3 hr, the virus infected cells showed a significantly different Venus/cyan fluorescent protein (CFP) ratio compared to the mock-infected cells. The ATeam technology is therefore useful to determine the early signs of ATP-based CPE as early as 3 hr without morphology-based CPE by light microscopy, and enables high throughput determination of the presence of microorganisms in neglected samples stored in laboratories.
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Doysabas et al. (2020) studied Viral infection (in vitro). ATeam technology (FRET-based ATP probe) vs. Mock-infected cells was evaluated on Venus/CFP fluorescence ratio (intracellular ATP level) (p=<0.01). ATeam technology detected early signs of viral cytopathic effect through significant changes in intracellular ATP levels as early as 3 hours post-infection, before morphological changes were visible.
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