Chemiluminescent immunoassay with two types of a multispot membrane strip has been developed for the simultaneous determination of several pesticides in water samples. Three types of antibodies with different cross‐reactivity towards s‐triazines were immobilized in three separate spots of the first strip (called a ‘triazine membrane strip') by means of a photoimmobilization technique. The second strip (called a ‘pesticide membrane strip') contained two pesticide‐specific spots with immobilized antibodies, one specific to 2,4‐dichlorophenoxyacetic acid and another with a high extent of cross‐reactivity toward different s‐triazines. Each membrane strip contained two additional spots. One was employed as a positive control or cut‐off spot, and the other one was used to check the background luminescence. The immunoassay with a multispot membrane strip allowed us to estimate semi‐quantitatively the concentration for a particular analyte by a comparison of the recorded light intensities of the analyte spot and the cut‐off spot. The application of the triazine membrane strip provides a differentiation between atrazine, simazine and terbuthylazine in mixtures, and the pesticide membrane strip allows the performance of group‐specific assays.
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Rubtsova et al. (1998) studied this question.
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