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The commitment phenomenon exhibited by Ceratocystis ulmi is a manifestation of the requirement for a Ca(II)—calmodulin interaction for mycelial growth. Under otherwise identical conditions, addition of CaCl2(10 mM) to committed yeasts caused them to germinate, while addition of the calmodulin antagonists chlorpromazine (80 µM), dibucaine (750 µM), or trifluoperazine (60 µM) to committed mycelia caused them to bud.
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Muthukumar et al. (1985) studied this question.
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