Isolation and characterization of Achromobacter sp. CX2 from symbiotic Cytophagales, a non-cellulolytic bacterium showing synergism with cellulolytic microbes by producing β-glucosidase

A Gram-negative, obligately aerobic, non-cellulolytic bacterium was isolated from the cellulolytic association of Cytophagales. It exhibits biochemical properties that are consistent with its classification in the genus Achromobacter . Phylogenetic analysis together with the phenotypic characteristics suggest that the isolate could be a novel species of the genus Achromobacter and designated as CX2 (= CGMCC 1.12675 = CICC 23807). The strain CX2 is the symbiotic microbe of Cytophagales and produces β-glucosidase. The results showed that the non-cellulolytic Achromobacter sp. CX2 has synergistic activity with cellulolytic microbes by producing β-glucosidase. To our knowledge, this is the first report on the synergetic effect of the combination of non-cellulolytic and cellulolytic microbes, which is significant to help understand the cellulolytic mechanism of cellulose-digesting Cytophagales.