Key result
Co-loading N-acetylcysteine into Fe3O4 nanoparticles mitigates iron-induced oxidative injury and apoptosis in cardiomyocytes.
Why the study?
Myocardial delivery of magnetic iron oxide nanoparticles may cause iron overload-induced myocardial injury via oxidative stress, prompting evaluation of antioxidant modification to mitigate toxicity.
Does co-loading N-acetylcysteine into iron oxide nanoparticles reduce cytotoxicity in neonatal rat hypoxia/reoxygenation cardiomyocytes?
Does co-loading N-acetylcysteine into iron oxide nanoparticles reduce cytotoxicity in neonatal rat hypoxia/reoxygenation cardiomyocytes?
Antioxidant modification with N-acetylcysteine reduces the in vitro cytotoxicity of iron oxide nanoparticles in cardiomyocytes, offering a potential strategy to improve their safety profile.
Antioxidant-modified MNPs merit in vivo testing for myocardial delivery; leaves open clinical translation from this in vitro data alone.
Purpose: Myocardial delivery of magnetic iron oxide nanoparticles (MNPs) might produce iron overload-induced myocardial injury, and the oxidative stress was regarded as the main mechanism. Therefore, we speculated antioxidant modification might be a reasonable strategy to mitigate the toxicity of MNPs. Methods and results: Antioxidant N-acetylcysteine (NAC) was loaded into magnetic mesoporous silica coated Fe 3 O 4 nanoparticles. Neonatal rat hypoxia/reoxygenation (H/R) cardiomyocytes were incubated with nanoparticles for 24 hrs. NAC can effectively mitigate iron-induced oxidative injury of cardiomyocytes, evidenced by reduced production of MDA, 8-iso-PGF2α, and 8-OHDG and maintained concentrations of SOD, CAT, GSH-Px, and GSH in ELISA and biochemical tests; downregulated expression of CHOP, GRP78, p62, and LC3-II proteins in Western Blot, and less cardiomyocytes apoptosis in flow cytometric analysis. Conclusions: NAC modifying could suppress the toxic effects of Fe 3 O 4 nanoparticles in H/R cardiomyocytes model in vitro, indicating a promising strategy to improve the safety of iron oxide nanoparticles.
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Shen et al. (2019) studied Hypoxia/reoxygenation myocardial injury. N-acetylcysteine (NAC) loaded into magnetic mesoporous silica coated Fe3O4 nanoparticles vs. Fe3O4 nanoparticles without NAC (implied) was evaluated on Oxidative injury and apoptosis. Co-loading antioxidant N-acetylcysteine into Fe3O4 nanoparticles effectively mitigated iron-induced oxidative injury and apoptosis in hypoxia/reoxygenation cardiomyocytes.
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