No restrictions on the sequence variety is one of the key features of the presented solid-phase synthesis for small- to medium-sized cyclic oligoribonucleotides (see diagram). The approach allows fully automated assembly with 2′-O-triisopropylsilyloxymethyl (TOM) RNA phosphoramidites. Ring closure is achieved by standard phosphotriester RNA chemistry. Selective cleavage from the solid support provides the cyclic oligoribonucleotide in high purity after deprotection.
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Ronald Micura (1999) studied this question.