The Protein Synthesis Dependent on the RNA Directed by Irradiated Calf Thymus DNA and Deoxyribonucleoprotein

The translational ability of RNA directed by irradiated calf thymus DNA or deoxyribonucleoprotein (DNP) in vitro was investigated. Both the template activity of calf thymus DNA for RNA synthesis and the incorporation of amino acids into acid insoluble materials in vitro markedly decreased by irradiation with 20 increasing dose. The activity of calf thymus DNP, however, increased with irradiation at a dose lower than krads. The incorporation of amino acids also increased with the same dose. It was shown that the translation dependent on the RNA directed by irradiated templates was qualitatively different from that of unirradiated templates.