An acetylcholine-like substance extracted from etiolated seedlings of Vigna sesquipedalis was purified by Sephadex G-15 column chromatography, paper chromatography and a newly devised spot test. It was identified as acetylcholine by field desorption mass spectrometry. Vigna acetylcholine also could be separated by the use of a liquid cation exchanger, sodium tetraphenylboron in n-butyronitrile.
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Toshiaki Hoshino (1983) studied this question.
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