Key result
Two multiplex qRT-PCR assays detect enteric viruses in sediment and shellfish down to 1 RNA copy/μL.
Population
Aquatic sediment and shellfish samples
Comparison
Two multiplex qRT-PCR assays targeting norovirus… vs Singleplex qRT-PCR assays
Authors
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Validated multiplex qRT-PCR assays enable sediment viral monitoring; extends surveillance tools yet leaves open public-health impact.
The developed multiplex qRT-PCR assays provide a highly sensitive and reliable method for estimating viral titres in environmental sediment and shellfish samples.
Farkas et al. (2017) studied Enteric virus contamination in environmental samples. Two triplex one-step qRT-PCR assays vs. Singleplex qRT-PCR assays was evaluated on Limit of detection (LOD) and limit of quantification (LOQ) for target viruses. Two multiplex qRT-PCR assays demonstrated high sensitivity for detecting enteric viruses in sediment and shellfish, with a limit of detection of 1 RNA copy/μL and limits of quantification of 3-18 RNA copies/μL.
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